大鼠肠黏膜的溶酶体酶

LYSOSOMAL ENZYMES OF RAT INTESTINAL MUCOSA.

作者信息

HSU L, TAPPEL A L

出版信息

J Cell Biol. 1964 Nov;23(2):233-40. doi: 10.1083/jcb.23.2.233.

DOI:10.1083/jcb.23.2.233

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2106522/

Abstract

Six intracellular hydrolases known to be associated with lysosomes in rat liver were found in rat intestinal mucosa. The extent to which they were particulate-bound and the degree of enzyme release when the particulate fractions were suspended in hypotonic media followed the same pattern in both mucosa and liver. The specific activities of the mucosa enzymes were either comparable to or slightly smaller than those of the liver enzymes. These results suggest that the mucosa hydrolases belong to lysosome-like particles. However, differential fractionation of the mucosa indicated that the particles from the mucosa sediment at lower centrifugal forces than do those from the liver and are more heterogeneous in size, bearing a closer resemblance to kidney lysosomes. Possible physiological functions of particulate-bound digestive enzymes in intestinal mucosa are discussed.

摘要

在大鼠肠黏膜中发现了六种已知与大鼠肝脏溶酶体相关的细胞内水解酶。它们与颗粒结合的程度以及当颗粒组分悬浮于低渗介质中时酶的释放程度，在黏膜和肝脏中遵循相同模式。黏膜酶的比活性与肝脏酶相当或略低。这些结果表明，黏膜水解酶属于溶酶体样颗粒。然而，对黏膜的分级分离表明，来自黏膜的颗粒在比来自肝脏的颗粒更低的离心力下沉淀，并且在大小上更具异质性，与肾溶酶体更为相似。文中讨论了肠黏膜中与颗粒结合的消化酶可能的生理功能。

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