大鼠小肠中芳基硫酸酯酶的发育
The development of arylsulphatase in the small intestine of the rat.
作者信息
Danovith S H, Laster L
出版信息
Biochem J. 1969 Sep;114(2):343-50. doi: 10.1042/bj1140343.
Abstract
- Arylsulphatase activity was measured in stomach, proximal and distal third of small intestine, colon, liver and kidney of foetal and neonatal Sprague-Dawley rats and Swiss mice, with nitrocatechol sulphate as substrate. 2. The specific activity in the distal small intestine, but not in the stomach, proximal small intestine or colon, increased about fourfold between 5 and 16 days after birth in both conventional and germ-free rats. 3. No comparable increase occurred in the distal small intestine of the mouse. 4. The specific activity of acid phosphatase in the distal small intestine of the rat rose only slightly when the arylsulphatase activity increased. 5. The pH optimum and Michaelis constant of arylsulphatase activity of the distal small intestine were similar for 1-day-old, 9-day-old and adult rats. 6. When extracts of distal small intestine of 1-day-old and 9-day-old rats were incubated together, the arylsulphatase activities were additive.
摘要
- 以硫酸硝基邻苯二酚为底物，测定了胎鼠及新生斯普拉格-道利大鼠和瑞士小鼠的胃、小肠近端和远端三分之一、结肠、肝脏和肾脏中的芳基硫酸酯酶活性。2. 在常规饲养和无菌饲养的大鼠中，出生后5至16天，远端小肠中的比活性增加了约四倍，而胃、小肠近端或结肠中的比活性未增加。3. 小鼠的远端小肠未出现类似的增加。4. 当大鼠远端小肠中的芳基硫酸酯酶活性增加时，酸性磷酸酶的比活性仅略有上升。5. 1日龄、9日龄和成年大鼠远端小肠中芳基硫酸酯酶活性的最适pH值和米氏常数相似。6. 将1日龄和9日龄大鼠远端小肠的提取物一起孵育时，芳基硫酸酯酶活性具有加和性。
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