使用三羟甲基氨基甲烷盐酸盐缓冲液用烟草花叶病毒接种番茄原生质体。
The use of tris-HCl buffer for inoculation of tomato protoplasts with tobacco mosaic virus.
作者信息
Motoyoshi F, Oshima N
出版信息
J Gen Virol. 1976 Aug;32(2):311-4. doi: 10.1099/0022-1317-32-2-311.
PMID:14223
Abstract
When tris-HCL was used as the buffer for inoculation of tomato protoplasts with tobacco mosaic virus, a greater proportion became infected than when phosphate was used. Using 0-05 M-tris-HCl buffer, pH 8-0, in the presence of poly-L-ornithine or poly-D-lysine, 50 to 80 % infection was obtained.
摘要
当使用三羟甲基氨基甲烷盐酸盐（tris-HCL）作为缓冲液，用于将烟草花叶病毒接种到番茄原生质体时，与使用磷酸盐相比，有更大比例的原生质体被感染。在聚-L-鸟氨酸或聚-D-赖氨酸存在的情况下，使用pH值为8.0的0.05M三羟甲基氨基甲烷盐酸盐（tris-HCL）缓冲液，可实现50%至80%的感染率。
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