Prakash J, Foxe M J
Arch Virol. 1985;85(3-4):269-79. doi: 10.1007/BF01314236.
Conditions for the reproducible infection of potato protoplasts with PVX were determined. Successful virus infection was achieved when potato protoplasts at a concentration of 3 X 10(5) cells/ml were incubated with 20 micrograms/ml PVX and 2 micrograms/ml Poly-L-ornithine (MW 100,000) for 15 minutes at 0 degrees C. Numbers of protoplasts infected varied between 30 and 50 per cent. Preincubation of PVX with PLO for a minimum of 10 minutes was essential for good infection. The type and molecular weight of polycation used had a major effect on virus infection. PLO at 100,000 MW was the most efficient.
确定了用PVX可重复性感染马铃薯原生质体的条件。当浓度为3×10⁵个细胞/毫升的马铃薯原生质体与20微克/毫升PVX和2微克/毫升聚-L-鸟氨酸（分子量100,000）在0℃下孵育15分钟时，可成功实现病毒感染。被感染的原生质体数量在30%至50%之间变化。PVX与聚-L-鸟氨酸预孵育至少10分钟对于良好感染至关重要。所用聚阳离子的类型和分子量对病毒感染有重大影响。分子量为100,000的聚-L-鸟氨酸效率最高。
