3,3'-二羟基-α,β-二乙基芪和3,3',4,5'-四羟基芪对体外微管组装、非整倍体诱导以及细胞微管和肌动蛋白网络的影响。

Effects of 3,3'-dihydroxy-alpha,beta-diethylstilbene and 3,3',4,5'-tetrahydroxystilbene on microtubule assembly in vitro, aneuploidy induction, and cellular microtubule and actin networks.

作者信息

Sakakibara Y, Saito I, Oda T, Tsujibo H, Inamori Y, Sato Y

机构信息

Kyoritsu College of Pharmacy, Tokyo, Japan.

出版信息

Chem Pharm Bull (Tokyo). 1992 Aug;40(8):2115-9. doi: 10.1248/cpb.40.2115.

DOI:10.1248/cpb.40.2115

PMID:1423765

Abstract

We examined the inhibitory activities of 3,3'-dihydroxy-alpha,beta-diethylstilbene (DDS) and 3,3',4,5'-tetrahydroxystilbene (THS) on microtubule assembly in vitro and their effects on chromosome number and cellular microtubule networks in Chinese hamster V79 cells. DDS showed half the inhibitory activity of diethylstilbestrol (DES) on microtubule assembly in vitro, while THS had none of the inhibitory activity. DDS induced tetraploid at 30 microM, whereas THS was found to be inactive. Furthermore, DDS disturbed cellular microtubule networks at 100 microM. We also examined the effects of DES, DDS and THS on cellular actin networks in mouse BALB 3T3 cells. DES induced a change of actin stress fiber distribution and THS had similar activity, while DDS showed no activity.

摘要

我们检测了3,3'-二羟基-α,β-二乙基芪（DDS）和3,3',4,5'-四羟基芪（THS）对体外微管组装的抑制活性，以及它们对中国仓鼠V79细胞染色体数目和细胞微管网络的影响。DDS在体外对微管组装的抑制活性是己烯雌酚（DES）的一半，而THS没有抑制活性。DDS在30微摩尔时诱导四倍体产生，而THS无此活性。此外，DDS在100微摩尔时扰乱细胞微管网络。我们还检测了DES、DDS和THS对小鼠BALB 3T3细胞中细胞肌动蛋白网络的影响。DES诱导肌动蛋白应力纤维分布改变，THS有类似活性，而DDS无活性。