Sato Y, Sakakibara Y, Oda T, Aizu-Yokota E, Ichinoseki K
Kyoritsu College of Pharmacy, Tokyo, Japan.
Chem Pharm Bull (Tokyo). 1992 Jan;40(1):182-4. doi: 10.1248/cpb.40.182.
The effects of estradiol (E2) and ethynylestradiol (EE2) on the chromosome number and cellular microtubule architecture of Chinese hamster V79 cells were studied using fluorescent anti-tubulin antibody. Treatment with 20 microM E2 for 48 h induced only a small amount of tetraploid cells, but the normal microtubule network was disrupted completely by only 3 h of treatment. This data reveals that E2 has higher microtubule-disruptive activity than diethylstilbestrol in V79 cells.
使用荧光抗微管蛋白抗体研究了雌二醇（E2）和乙炔雌二醇（EE2）对中国仓鼠V79细胞染色体数目和细胞微管结构的影响。用20微摩尔E2处理48小时仅诱导出少量四倍体细胞，但仅处理3小时正常微管网络就被完全破坏。该数据表明，在V79细胞中，E2比己烯雌酚具有更高的微管破坏活性。
