Clinch T E, Hobden J A, Hill J M, O'Callaghan R J, Engel L S, Kaufmann H E
LSU Eye Center, New Orleans 70112-2234.
CLAO J. 1992 Oct;18(4):245-7.
We compared collagen shields hydrated in 1.36% tobramycin with topical 1.36% tobramycin for sustained treatment of experimental Pseudomonas keratitis in rabbits. Antibiotic therapy for a total of 24 hours was initiated 14 hours after an intrastromal injection of 10(3) logarithmic phase Pseudomonas aeruginosa. Seven groups were treated as follows: groups 1-3, collagen shields hydrated in tobramycin supplemented with topical 1.36% tobramycin drops at 4, 6, or 8 hour intervals; group 4, collagen shields hydrated in tobramycin without any further topical supplementation; group 5, topical tobramycin therapy, initially every half-hour for 4 hours, then hourly; group 6, collagen shields hydrated in balanced saline solution; and group 7, no treatment. Each group contained four eyes. The groups treated with collagen shields supplemented every 4 or 6 hours with topical tobramycin had significantly fewer colony forming units (CFU) than those receiving topical or collagen shield therapy alone (P < 0.001). The group treated with collagen shields hydrated in sterile saline had 10(7) CFU per cornea, which was not significantly different from the untreated group (P > 0.2). Collagen shields hydrated in tobramycin and supplemented with topical tobramycin were effective in sustained treatment of experimental Pseudomonas keratitis.
我们比较了用1.36%妥布霉素水合的胶原盾与局部使用1.36%妥布霉素对兔实验性铜绿假单胞菌角膜炎的持续治疗效果。在基质内注射10³对数期铜绿假单胞菌14小时后开始总共24小时的抗生素治疗。七组的治疗方式如下：第1 - 3组，用妥布霉素水合的胶原盾，并每隔4、6或8小时补充局部1.36%妥布霉素滴眼液；第4组，用妥布霉素水合的胶原盾，不再进行任何进一步的局部补充；第5组，局部妥布霉素治疗，最初每半小时一次，共4小时，然后每小时一次；第6组，用平衡盐溶液水合的胶原盾；第7组，不治疗。每组有四只眼睛。每4或6小时补充局部妥布霉素的胶原盾治疗组的菌落形成单位（CFU）明显少于单独接受局部或胶原盾治疗的组（P < 0.001）。用无菌盐水水合的胶原盾治疗组每只角膜有10⁷CFU，与未治疗组无显著差异（P > 0.2）。用妥布霉素水合并补充局部妥布霉素的胶原盾对兔实验性铜绿假单胞菌角膜炎的持续治疗有效。
