Hobden J A, Reidy J J, O'Callaghan R J, Insler M S, Hill J M
Department of Microbiology, Louisiana State University Medical Center School of Medicine, New Orleans.
Invest Ophthalmol Vis Sci. 1990 Nov;31(11):2241-3.
Rabbit corneas were infected with a tobramycin-resistant (minimum inhibitory concentration, 31.25 micrograms/ml) strain of Pseudomonas aeruginosa 27853 (10(3) colony-forming units) and were treated 22 hours later with collagen corneal shields hydrated in either 25 mg/ml ciprofloxacin, 40 mg/ml norfloxacin, 40 mg/ml tobramycin, or deionized water. Shields were removed at 26 hours postinfection, and 1 hour later, corneas were harvested for bacterial enumeration. Application of shields hydrated in ciprofloxacin reduced the number of viable bacteria per cornea approximately 4 log units compared with the application of shields containing tobramycin or deionized water (P less than 0.0001). Use of shields hydrated in norfloxacin reduced the number of P. aeruginosa organisms by greater than 2 log units compared with shields containing tobramycin or deionized water (P less than 0.0001). Ciprofloxacin was significantly more effective than norfloxacin in reducing the number of bacteria per cornea (P less than 0.0001). There was no significant difference in the number of bacteria recovered from corneas treated with tobramycin or deionized water (P less than 0.56).
用对妥布霉素耐药（最低抑菌浓度为31.25微克/毫升）的铜绿假单胞菌27853菌株（10³ 菌落形成单位）感染兔角膜，22小时后用在25毫克/毫升环丙沙星、40毫克/毫升诺氟沙星、40毫克/毫升妥布霉素或去离子水中水化的胶原角膜罩进行治疗。在感染后26小时移除角膜罩，1小时后，采集角膜进行细菌计数。与使用含妥布霉素或去离子水的角膜罩相比，使用在环丙沙星中水化的角膜罩可使每个角膜的活菌数减少约4个对数单位（P<0.0001）。与使用含妥布霉素或去离子水的角膜罩相比，使用在诺氟沙星中水化的角膜罩可使铜绿假单胞菌数量减少超过2个对数单位（P<0.0001）。在减少每个角膜细菌数量方面，环丙沙星比诺氟沙星显著更有效（P<0.0001）。用妥布霉素或去离子水处理的角膜回收的细菌数量没有显著差异（P<0.56）。
