Dolwick M F, Bush F M, Seibel H R
Acta Anat (Basel). 1977;98(3):325-33. doi: 10.1159/000144808.
The histoenzymatic characteristics of regenerating myofibers of rat masseter muscle following injection of 1% lidocaine, as well as morphometric and histochemical characteristics of the typical myofibers, were investigated. Myoblasts appeared initially by day 1 among numerous macrophages within the confines of degenerating myofibers. Myotubes predominated by the 3rd day. Complete regeneration of the muscle occurred by at least 45 days. Phosphorylase activity was absent at day 1 and reappeared by the 5th day when the regenerating myofibers showed slight activity. By the 15th day the myofiber types had partly differentiated; red myofibers were smaller and stained less intensely than the white myofibers. Myotubes stained uniformly for succinic dehydrogenase activity from 3 until 5 days. After 5 days this staining increased gradually. Myofiber types began differentiation by 15 days and were fully differentiated by 45 days. ATPase activity was barely evident by 1-3 days. This activity appeared uniformly low up to 5 days and increased to an intensity comparable with that of the typical myofiber by 15 days. Slight leucine aminopeptidase activity occurred in macrophages 1 day following injection. By 3 days this activity appeared in the remaining myoblasts and in the myotubes. Some activity was found in the fibroblasts. This staining intensity at 5 days was equal to that of earlier lesions. A trace of this activity was found at 7 days, and none at 15 days. Glucose-6-phosphate dehydrogenase activity was present in the macrophages by day 1. It increased by 3 days and occurred mainly in myoblasts and myotubes. This activity decreased by 5 days, and none was found by 7 days.
研究了注射1%利多卡因后大鼠咬肌再生肌纤维的组织酶学特征，以及典型肌纤维的形态计量学和组织化学特征。成肌细胞最初在第1天出现在退变肌纤维范围内的大量巨噬细胞中。到第3天，肌管占主导。肌肉至少在45天时完全再生。第1天时磷酸化酶活性缺失，到第5天时重新出现，此时再生肌纤维显示出轻微活性。到第15天时，肌纤维类型部分分化；红色肌纤维比白色肌纤维小且染色强度较低。肌管在第3天至第5天琥珀酸脱氢酶活性染色均匀。5天后这种染色逐渐增加。肌纤维类型在15天时开始分化，45天时完全分化。1至3天时ATP酶活性几乎不明显。直到5天时这种活性一直均匀较低，到15天时增加到与典型肌纤维相当的强度。注射后1天，巨噬细胞中出现轻微的亮氨酸氨肽酶活性。到第3天时，这种活性出现在剩余的成肌细胞和肌管中。在成纤维细胞中也发现了一些活性。5天时这种染色强度与早期损伤时相同。7天时发现微量这种活性，15天时未发现。第1天时巨噬细胞中存在葡萄糖-6-磷酸脱氢酶活性。到第3天时增加，主要出现在成肌细胞和肌管中。这种活性在5天时下降，7天时未发现。
