两栖动物（非洲爪蟾、日本林蛙）组织中D-天冬氨酸氧化酶和游离D-天冬氨酸的存在

Presence of D-aspartate oxidase and free D-aspartate in amphibian (Xenopus laevis, Cynops pyrrhogaster) tissues.

Kera Y, Nagasaki H, Iwashima A, Yamada R

Department of BioEngineering, Nagaoka University of Technology, Japan.

Comp Biochem Physiol B. 1992 Oct;103(2):345-8. doi: 10.1016/0305-0491(92)90302-8.

Abstract

This paper is the first report on the presence of D-aspartate oxidase activity and free D-aspartate in the amphibian tissues. 2. The presence of D-aspartate oxidase activity in tissues of clawed toad (Xenopus laevis) and Japanese newt (Cynops pyrrhogaster) was demonstrated by requirements for enzyme activity, selective inhibition with meso-tartrate and substrate specificity. 3. In each animal, the highest activity was found in kidney, followed by liver and brain, and no gender difference in the specific activity was observed in each tissue. 4. A small but significant amount of D-aspartate was detected in liver and kidney, irrespective of species. 5. In the newt, there was a gender difference in the hepatic and renal content of D-aspartate and not in the D-/D+L-aspartate ratio.

摘要

本文首次报道了两栖动物组织中存在D - 天冬氨酸氧化酶活性和游离D - 天冬氨酸。2. 通过酶活性需求、内消旋酒石酸盐的选择性抑制以及底物特异性，证明了爪蟾（非洲爪蟾）和日本蝾螈（红腹蝾螈）组织中存在D - 天冬氨酸氧化酶活性。3. 在每只动物中，肾脏中的活性最高，其次是肝脏和大脑，并且在每个组织中均未观察到比活性的性别差异。4. 无论物种如何，在肝脏和肾脏中均检测到少量但显著量的D - 天冬氨酸。5. 在蝾螈中，肝脏和肾脏中D - 天冬氨酸的含量存在性别差异，而D - /D + L - 天冬氨酸比率不存在性别差异。