FORSHAW J, HARWOOD L
J Clin Pathol. 1965 Jan;18(1):119-20. doi: 10.1136/jcp.18.1.119.
Folic acid deficiency with the picture of a megaloblastic bone marrow may develop in haemolytic anaemia, and, on the other hand, both vitamin B(12) and folic acid deficiency may produce signs of haemolysis. As the correct interpretation of a positive antiglobulin reaction associated with megaloblastic erythropoiesis is particularly important, the effect of deficiency of vitamin B(12) and folic acid on the results of the test was investigated in 32 patients with vitamin B(12) or folic acid deficiency and a positive antiglobulin reaction was obtained in ten. There was no correlation between the result of the test and the degree of anaemia, and there was no significant difference between the incidence of positive results associated with deficiency of vitamin B(12) or folic acid. In determining the significance of a positive result, the time interval before agglutination occurs is sometimes of greater value than the strength of the reaction or the result of the gamma globulin neutralization test.
溶血性贫血可能会出现伴有巨幼细胞性骨髓象的叶酸缺乏，另一方面，维生素B₁₂和叶酸缺乏都可能产生溶血迹象。由于正确解读与巨幼细胞性红细胞生成相关的阳性抗球蛋白反应尤为重要，因此对32例维生素B₁₂或叶酸缺乏且抗球蛋白反应呈阳性的患者，研究了维生素B₁₂和叶酸缺乏对检测结果的影响，其中10例获得了阳性抗球蛋白反应。检测结果与贫血程度之间无相关性，维生素B₁₂或叶酸缺乏相关的阳性结果发生率之间也无显著差异。在确定阳性结果的意义时，凝集发生前的时间间隔有时比反应强度或γ球蛋白中和试验结果更有价值。
