METZGER H, MANNIK M
J Exp Med. 1964 Nov 1;120(5):765-82. doi: 10.1084/jem.120.5.765.
Conditions were developed by which the separated H and L chains of gamma(2) globulins recombined to form four-chained molecules in good yields. In the absence of antigen, anti-2,4-dinitrophenyl (anti-DNP) H chains randomly reassociated with a mixture of antibody and non-specific gamma(2) globulin L chains. In the presence of a specific hapten, however, the antibody H chains preferentially interacted with the anti-DNP L chains. Antibody H chain-antibody L chain recombinants formed in the presence of hapten were more active than the corresponding recombinants formed in the absence of hapten. Speculations are made regarding the possible mechanisms and biological significance of these effects.
已建立了相关条件,在这些条件下,γ(2)球蛋白的分离重链和轻链能够以较高产率重新组合形成四链分子。在没有抗原的情况下,抗2,4-二硝基苯基(抗DNP)重链与抗体和非特异性γ(2)球蛋白轻链的混合物随机重新结合。然而,在存在特定半抗原的情况下,抗体重链优先与抗DNP轻链相互作用。在半抗原存在下形成的抗体重链-抗体轻链重组体比在没有半抗原的情况下形成的相应重组体更具活性。对这些效应的可能机制和生物学意义进行了推测。