Hollister J R, Mannik M
Clin Exp Immunol. 1974 Apr;16(4):615-27.
In the rabbit model of antigen-induced arthritis the duration and mechanism of antigen retention were investigated. The tissue half-life of retained antigen in descending order was meniscus (141 days), articular cartilage (31 days), ligaments (17 days), synovium (8 days) and synovial fluid (4 days). The retention of antigen in collagenous tissue was very firm. Perturbant solutions were used to study the binding of antigen to menisci, and only 6 M guanidine was effective in removing the antigen. The role of specific antibody was investigated. Antibody was bound to normal menisci, but the Fc portion of the molecule was not critical to the uptake of antibody by collagenous tissue. Finally, the interaction of antigen, antibody, and collagenous tissue was studied. Preformed soluble immune complexes produced three times the retention of antigen alone. Sequential administration of antibody and antigen yielded fifteen times the retention of antigen in the normal rabbit. Therefore, antigen retention in collagenous tissue was due to immune complex formation with specific antibody within the cartilage matrix.
在抗原诱导性关节炎的兔模型中，研究了抗原保留的持续时间和机制。保留抗原在组织中的半衰期由长到短依次为半月板（141天）、关节软骨（31天）、韧带（17天）、滑膜（8天）和滑液（4天）。抗原在胶原组织中的保留非常牢固。使用扰动溶液研究抗原与半月板的结合，只有6M胍能有效去除抗原。研究了特异性抗体的作用。抗体与正常半月板结合，但分子的Fc部分对胶原组织摄取抗体并不关键。最后，研究了抗原、抗体和胶原组织之间的相互作用。预先形成的可溶性免疫复合物使抗原的保留量比单独抗原增加了两倍。在正常兔中依次给予抗体和抗原，抗原的保留量增加了15倍。因此，胶原组织中的抗原保留是由于在软骨基质内与特异性抗体形成免疫复合物所致。
