Katz D H, Paul W E, Goidl E A, Benacerraf B
J Exp Med. 1970 Aug 1;132(2):261-82. doi: 10.1084/jem.132.2.261.
Preimmunization of either guinea pigs or rabbits to bovine gamma globulin (BGG) prepares the animals for markedly enhanced antibody responses to 2,4-dinitrophenyl-BGG (DNP-BGG). This phenomenon is observed both in the primary anti-DNP antibody response to DNP-BGG and in the secondary anti-DNP antibody response to DNP-BGG in animals primed with DNP-ovalbumin (DNP-OVA). The BGG preimmunization is most effective if the antigen is administered as a complete Freund's adjuvant emulsion; in rabbits, a dose of 1 microg of BGG is more effective than a dose of 50 microg, whereas the reverse is true in guinea pigs. Transfusion of homologous anti-BGG sera fails to replace active immunization with BGG in the preparation of animals for these enhanced anti-DNP antibody responses. Both the immunoglobulin class and the average association constant for epsilon-DNP-L-lysine of the anti-DNP antibody produced in these enhanced responses is determined by the mode and time of immunization with haptenic conjugates and is not appreciably influenced by the nature of the carrier preimmunization. These studies indicate that the carrier specificity of hapten-specific anamnestic antibody responses is largely due to the interaction of two independent cell associated recognition units, one specialized for carrier and the other specific for haptenic determinants.
用牛γ球蛋白（BGG）对豚鼠或兔子进行预免疫，可使动物对2,4 -二硝基苯基 - BGG（DNP - BGG）的抗体反应显著增强。在对DNP - BGG的初次抗DNP抗体反应以及用DNP - 卵清蛋白（DNP - OVA）致敏的动物对DNP - BGG的二次抗DNP抗体反应中，均观察到这种现象。如果将抗原作为完全弗氏佐剂乳剂给药，BGG预免疫最为有效；在兔子中，1微克BGG的剂量比50微克的剂量更有效，而在豚鼠中则相反。输注同源抗BGG血清无法替代用BGG进行主动免疫，以让动物对这些增强的抗DNP抗体反应做好准备。在这些增强反应中产生的抗DNP抗体的免疫球蛋白类别以及针对ε - DNP - L - 赖氨酸的平均结合常数，由用半抗原共轭物免疫的方式和时间决定，且不受载体预免疫性质的明显影响。这些研究表明，半抗原特异性回忆性抗体反应的载体特异性很大程度上是由于两个独立的细胞相关识别单元相互作用所致，一个专门针对载体，另一个针对半抗原决定簇。
