WILSNACK R E, ROWE W P
J Exp Med. 1964 Nov 1;120(5):829-40. doi: 10.1084/jem.120.5.829.
LCM virus antigen was visualized in infected tissue cultures and mouse tissues by the direct immunofluorescent procedure. In all instances, antigen was localized to the cytoplasm. In intracerebrally infected mice, antigen was almost completely restricted to meninges, choroid plexus, and ependyma; at no time were neurons involved. Mice infected by intraperitoneal inoculation of a viscerotropic strain demonstrated antigen chiefly in liver parenchyma, splenic reticulum, bronchi, and alveolar cells. Congenitally infected mice showed antigen in almost all cell types observed, but generally in only a minority of cells; infection was often focal in distribution. Liver, kidney, pregnant uterus, and trophoblast were most extensively involved. No antigen was observable in ova of a pregnant mouse.
通过直接免疫荧光法在感染的组织培养物和小鼠组织中观察到淋巴细胞脉络丛脑膜炎病毒抗原。在所有情况下,抗原都定位于细胞质中。在脑内感染的小鼠中,抗原几乎完全局限于脑膜、脉络丛和室管膜;神经元从未受累。通过腹腔接种嗜内脏毒株感染的小鼠,抗原主要出现在肝实质、脾网状组织、支气管和肺泡细胞中。先天性感染的小鼠在几乎所有观察到的细胞类型中都显示有抗原,但通常仅在少数细胞中出现;感染分布往往呈局灶性。肝脏、肾脏、妊娠子宫和滋养层受累最为广泛。在妊娠小鼠的卵子中未观察到抗原。