COONS A H, KAPLAN M H
J Exp Med. 1950 Jan 1;91(1):1-13. doi: 10.1084/jem.91.1.1.
Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.
本文描述了一种用于组织细胞中抗原特异性显微定位方法的改进。该方法采用异硫氰酸荧光素标记的抗体作为组织化学染色剂,在荧光显微镜下可观察到特异性抗原-抗体沉淀物。文中还描述了由硝基荧光素衍生的两个异构系列。