Rohrbach R, Iversen O H, Riede U N, Sandritter W
Beitr Pathol. 1977 May;160(2):175-86. doi: 10.1016/s0005-8165(77)80023-1.
Variations in epidermal chalones after a single surface application of methylcholanthrene and croton oil have been described in previous papers. This paper reports a study of the effect of adhesive tape stripping of the skin on epidermal growth regulators (G1 and G2 chalones). Pieces of adhesive tape were applied 6 times to the same area of skin in groups of mice. The short-term effects of tape stripping on epidermal DNA synthesis and on mitotic rate were studied at different intervals after stripping. Other groups of mice were killed at similar time intervals after stripping, and the treated area of skin was homogenized and extracted with water. The inhibitory effect of these extracts on normal epidermal DNA synthesis and mitotic rate was assayed in normal hairless mice. The resulting inhibitions were interpreted as an expression of the concentration of G1 or G2 chalone in the skin extracts. The first experiment confirmed that cellophane tape stripping gives rise to a short block in epidermal mitotic activity and probably also in DNA synthesis. This was followed by bimodal peaks of increased activity, the two maxima of labelling index being found on days 2 and 6, and the two maxima of mitotic rate on days 1-2 and 7. The concentrations of the two chalones in the skins of treated animals varied in inverse proportion to the alterations in the DNA synthesis and the mitotic rate, with one exception. Here there was initially a depression of the mitotic rate and a low concentration of G2 chalone. This was interpreted as a short reaction of the basel cells to the stripping trauma. It is concluded that adhesive tape stripping removes the differentiating cells and injures some basel cells, simultaneously altering the content of G1 and G2 chalones. The resulting increase in the rates of DNA synthesis and mitosis lasts only until the number of cells is high enough to produce growth-regulating substances (chalones) again. This theory may explain the changes observed. Since similar reactions are seen after both carcinogenic and co-carcinogenic chemical injury of the epidermis, the reaction pattern seems to be a general response to cell injury or cell removal.
在先前的论文中已经描述了单次表面涂抹甲基胆蒽和巴豆油后表皮抑素的变化。本文报道了一项关于皮肤胶带剥离对表皮生长调节因子（G1和G2抑素）影响的研究。将胶带片在小鼠组的同一皮肤区域粘贴6次。在剥离后的不同时间间隔研究胶带剥离对表皮DNA合成和有丝分裂率的短期影响。其他小鼠组在剥离后的相似时间间隔处死，将处理过的皮肤区域匀浆并用水提取。在正常无毛小鼠中测定这些提取物对正常表皮DNA合成和有丝分裂率的抑制作用。所产生的抑制作用被解释为皮肤提取物中G1或G2抑素浓度的一种表达。第一个实验证实玻璃纸胶带剥离导致表皮有丝分裂活性短暂阻滞，可能也导致DNA合成阻滞。随后是活性增加的双峰，标记指数的两个最大值出现在第2天和第6天，有丝分裂率的两个最大值出现在第1 - 2天和第7天。处理过的动物皮肤中两种抑素的浓度与DNA合成和有丝分裂率的变化成反比，但有一个例外。这里最初有丝分裂率降低且G2抑素浓度较低。这被解释为基底细胞对剥离创伤的短期反应。结论是胶带剥离去除了分化细胞并损伤了一些基底细胞，同时改变了G1和G2抑素的含量。所导致的DNA合成和有丝分裂率的增加仅持续到细胞数量足够多以再次产生生长调节物质（抑素）。该理论可能解释所观察到的变化。由于在表皮的致癌和促癌化学损伤后都能看到类似反应，这种反应模式似乎是对细胞损伤或细胞去除的一种普遍反应。
