Iversen O H
Virchows Arch B Cell Pathol. 1978 May 19;27(3):229-35.
Balb/c/nu nude mice that had been transplanted with a moderately differentiated squamous cell carcinoma were injected i.p. with different doses of epidermal chalone, and control animals were injected with saline. The labelling indices (H3TdR) and the mitotic rate (stathmokinetic method with vinblastine sulphate) were determined. In the untreated animals, both the labelling index and the mitotic rate of the tumor were considerably higher than in the epidermis, and the rate of cell birth was almost twice that of the epidermis. Higher doses of chalone were needed to reduce the labelling index for the tumour than for the epidermis, and there was generally a less pronounced dose/response relationship in the tumours than in the epidermis. The same was true of the mitotic rate but here the results were not as obvious as for the labelling index. A possible explanation of the results may be that the tumour cells are less sensitive than epidermal cells to the injected chalones, or that reduced vascularization of the transplanted tumour may lead to reduced access of chalone, or that tumour necrosis may pay a role. However, it is evident that the tumour cells react less than the epidermis to both the G1 and the G2 chalone, and thus the findings of this study do not provide any evidence against the theory that epidermoid transplanted tumours are less sensitive to epidermal chalones than normal tissue of the same histogenetic origin.
将中度分化的鳞状细胞癌移植到Balb/c/nu裸鼠体内后，经腹腔注射不同剂量的表皮抑素，对照组动物注射生理盐水。测定标记指数（H3TdR）和有丝分裂率（用硫酸长春碱的静止动力学方法）。在未经处理的动物中，肿瘤的标记指数和有丝分裂率均显著高于表皮，细胞生成率几乎是表皮的两倍。与表皮相比，需要更高剂量的抑素才能降低肿瘤的标记指数，而且肿瘤中的剂量/反应关系通常不如表皮明显。有丝分裂率也是如此，但此处结果不如标记指数明显。对这些结果的一种可能解释是，肿瘤细胞对注射的抑素不如表皮细胞敏感，或者移植肿瘤的血管化减少可能导致抑素的进入减少，或者肿瘤坏死可能起了作用。然而，很明显，肿瘤细胞对G1期和G2期抑素的反应都比表皮细胞小，因此本研究结果并未提供任何证据反驳表皮样移植肿瘤比相同组织发生学来源的正常组织对表皮抑素更不敏感这一理论。
