Takayama K, Nagata N, Miyagawa Y, Hirano H, Shigematsu N
Research Institute for Diseases of the Chest, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Chest. 1992 Nov;102(5):1441-3. doi: 10.1378/chest.102.5.1441.
To evaluate the usefulness of step sectioning of the transbronchial lung biopsy (TBLB) specimens obtained from patients with suspected sarcoidosis, we examined all TBLB specimens obtained from 132 patients who were diagnosed clinically as having sarcoidosis at our institute. When routine sections of TBLB specimens did not show sarcoid granuloma, we prepared additional serial sections from each block and stained every fifth section with hematoxylineosin (step sectioning). All step sections were examined histologically. With the aid of step sectioning, the diagnostic yield of sarcoidosis increased from 38 percent (18/47) to 47 percent (22/47) in stage 1 patients, and from 57 percent (37/65) to 82 percent (53/65) in stage 2 patients. All of the newly detected granulomas were identified between the first and seventh sections. We conclude that step sectioning is useful method in diagnosing sarcoidosis.
为评估对疑似结节病患者获取的经支气管肺活检（TBLB）标本进行阶梯切片的效用，我们检查了我院132例临床诊断为结节病患者的所有TBLB标本。当TBLB标本的常规切片未显示结节病肉芽肿时，我们从每个组织块制备额外的连续切片，并用苏木精 - 伊红对每第五张切片进行染色（阶梯切片）。对所有阶梯切片进行组织学检查。借助阶梯切片，1期患者结节病的诊断率从38%（18/47）提高到47%（22/47），2期患者从57%（37/65）提高到82%（53/65）。所有新检测到的肉芽肿均在第一张至第七张切片之间被识别。我们得出结论，阶梯切片是诊断结节病的一种有用方法。
