与酶解敏感性降低相关的聚-β-羟基丁酸颗粒的形态变化

MORPHOLOGICAL CHANGES IN POLY-BETA-HYDROXYBUTYRATE GRANULES ASSOCIATED WITH DECREASED SUSCEPTIBILITY TO ENZYMATIC HYDROLYSIS.

作者信息

MERRICK J M, LUNDGREN D G, PFISTER R M

出版信息

J Bacteriol. 1965 Jan;89(1):234-9. doi: 10.1128/jb.89.1.234-239.1965.

DOI:10.1128/jb.89.1.234-239.1965

PMID:14255669

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC315574/

Abstract

Merrick, J. M. (State University of New York, Buffalo), D. G. Lundgren, and R. M. Pfister. Morphological changes in poly-beta-hydroxybutyrate granules associated with decreased susceptibility to enzymatic hydrolysis. J. Bacteriol. 89:234-239. 1965.-A complex enzyme system obtained from extracts of Rhodospirillum rubrum cells hydrolyzes poly-beta-hydroxybutyric acid (PHB) contained in native PHB granules isolated from Bacillus megaterium. A labile factor associated with the granules and necessary for depolymerization is easily destroyed by various chemical and physical treatments. Granules inactivated by these treatments were examined in an electron microscope. In all cases, the distinct morphological appearance of native granules was altered. Morphological changes were mainly characterized by membrane fragmentation, loss of coalescence, and surface alterations. These observations suggest that native PHB granules possess definite structural features, disruption of which results in decreased susceptibility of the polymer to enzymatic hydrolysis.

摘要

梅里克，J.M.（纽约州立大学布法罗分校），D.G.伦德格伦，以及R.M.普费斯特。与酶促水解敏感性降低相关的聚-β-羟基丁酸酯颗粒的形态变化。《细菌学杂志》89:234 - 239。1965年。——从红螺菌细胞提取物中获得的一种复合酶系统可水解从巨大芽孢杆菌中分离出的天然聚-β-羟基丁酸酯（PHB）颗粒中所含的聚-β-羟基丁酸（PHB）。与颗粒相关且解聚所必需的一种不稳定因子很容易被各种化学和物理处理破坏。对经这些处理失活的颗粒进行了电子显微镜检查。在所有情况下，天然颗粒独特的形态外观都发生了改变。形态变化主要表现为膜破碎、失去聚结以及表面改变。这些观察结果表明，天然PHB颗粒具有确定的结构特征，其破坏会导致聚合物对酶促水解的敏感性降低。