Dunlop W F, Robards A W
J Bacteriol. 1973 Jun;114(3):1271-80. doi: 10.1128/jb.114.3.1271-1280.1973.
The freeze-etching technique was used to examine the effects of fracturing and etching on the appearance of poly-beta-hydroxybutyrate granules from Bacillus cereus. These granules were examined in extracts isolated by hypochlorite or by sonic treatment, and also in fixed and unfixed intact cells; in the latter case they were compared with granules in thin sections of intact cells. After freeze-fracturing, the diameter of the granules in intact cells was between 240 and 720 nm. The granules consisted of a central core, of diameter between 140 and 370 nm, which occupied less than 50% of the volume of the granule and which was either stretched or removed on fracturing; the core was surrounded by an outer coat which may be bounded by a membrane.
采用冷冻蚀刻技术研究断裂和蚀刻对蜡样芽孢杆菌聚-β-羟基丁酸酯颗粒外观的影响。这些颗粒在通过次氯酸盐或超声处理分离的提取物中进行检查，也在固定和未固定的完整细胞中进行检查；在后一种情况下，将它们与完整细胞薄片中的颗粒进行比较。冷冻断裂后，完整细胞中颗粒的直径在240至720纳米之间。颗粒由一个中心核组成，直径在140至370纳米之间，占颗粒体积的不到50%，在断裂时要么被拉伸要么被去除；核心被一层外层包围，外层可能由膜界定。
