从不同细菌中提取的聚-β-羟基丁酸酯的特性分析

CHARACTERIZATION OF POLY-BETA-HYDROXYBUTYRATE EXTRACTED FROM DIFFERENT BACTERIA.

作者信息

LUNDGREN D G, ALPER R, SCHNAITMAN C, MARCHESSAULT R H

出版信息

J Bacteriol. 1965 Jan;89(1):245-51. doi: 10.1128/jb.89.1.245-251.1965.

DOI:10.1128/jb.89.1.245-251.1965

PMID:14255672

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC315576/

Abstract

Lundgren, D. G. (Syracuse University, Syracuse, N.Y.), R. Alper, C. Schnaitman and R. H. Marchessault. Characterization of poly-beta-hydroxybutyrate extracted from different bacteria. J. Bacteriol. 89:245-251. 1965.-Poly-beta-hydroxybutyrate (PHB) from different bacterial genera was studied with regard to its crystal structure, infrared absorption, intrinsic viscosity, and electron microscopy. All PHB samples precipitated from dilute chloroform solution gave identical X-ray diffractograms confirming uniformity of crystal structure, and uniformity of molecular structure, based on the similarity of the recorded infrared spectra, was also established. Crystal morphology was also similar, showing the reported "lath" shape structure for purified polymer from Bacillus cereus. Intrinsic viscosity ranged from 0.04 to 11.5 depending upon the polymer treatment; polymer molecular weights, based upon viscometry, could be estimated to range from 1,000 to 250,000. It is concluded that the same basic molecule is involved in all PHB present in the bacterial kindgom.

摘要

伦德格伦，D. G.（纽约州锡拉丘兹市锡拉丘兹大学），R. 阿尔珀，C. 施奈特曼和R. H. 马尔切索尔特。从不同细菌中提取的聚-β-羟基丁酸酯的特性。《细菌学杂志》89:245 - 251。1965年。——对来自不同细菌属的聚-β-羟基丁酸酯（PHB）的晶体结构、红外吸收、特性粘度和电子显微镜进行了研究。从稀氯仿溶液中沉淀出的所有PHB样品给出了相同的X射线衍射图，证实了晶体结构的一致性，并且基于所记录的红外光谱的相似性，分子结构的一致性也得到了确立。晶体形态也相似，显示出蜡状芽孢杆菌纯化聚合物所报道的“板条”状结构。特性粘度根据聚合物处理的不同在0.04至11.5之间；基于粘度法，可以估计聚合物分子量在1000至250000之间。得出的结论是，细菌界中所有的PHB都涉及相同的基本分子。