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转化脱氧核糖核酸进入枯草芽孢杆菌过程中的构型

CONFIGURATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID DURING ENTRY INTO BACILLUS SUBTILIS.

作者信息

STRAUSS N

出版信息

J Bacteriol. 1965 Feb;89(2):288-93. doi: 10.1128/jb.89.2.288-293.1965.

Abstract

Strauss, Norman (Yale University, New Haven, Conn.). Configuration of transforming deoxyribonucleic acid during entry into Bacillus subtilis. J. Bacteriol. 89:288-293. 1965.-A correlation was obtained between map distance and the length of the lag period preceding the appearance of pairs of genetic traits after the addition of deoxyribonucleic acid (DNA) to a competent culture of Bacillus subtilis. The results are taken to indicate that DNA enters competent cells in lengthwise fashion. The smallest length of transforming DNA which can participate in a recombination event, and the number of nucleotide pairs which enter the cell per unit time, have been estimated. The evidence indicates that only part of the lag period is devoted to the transport of DNA into the cell. The significance of these results with respect to the mechanism of entry of DNA into the cell is discussed.

摘要

施特劳斯,诺曼(耶鲁大学,康涅狄格州纽黑文)。转化脱氧核糖核酸进入枯草芽孢杆菌过程中的构型。《细菌学杂志》89:288 - 293。1965年。——在向枯草芽孢杆菌感受态培养物中添加脱氧核糖核酸(DNA)后,图谱距离与成对遗传性状出现之前的延迟期长度之间获得了相关性。结果表明DNA以纵向方式进入感受态细胞。已经估计了能够参与重组事件的转化DNA的最小长度以及每单位时间进入细胞的核苷酸对数量。证据表明延迟期只有部分用于DNA向细胞内的运输。讨论了这些结果对于DNA进入细胞机制的意义。

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