枯草芽孢杆菌感受态细胞中脱氧核糖核酸摄取位点的数量。
Number of deoxyribonucleic acid uptake sites in competent cells of Bacillus subtilis.
作者信息
Singh R N
出版信息
J Bacteriol. 1972 Apr;110(1):266-72. doi: 10.1128/jb.110.1.266-272.1972.
Abstract
Two direct methods are presented for estimating the average number of deoxyribonucleic acid (DNA) uptake sites in competent cells of Bacillus subtilis from measurement of (14)C- or (3)H-thymine-labeled DNA uptake by competent culture. Advantage is taken of two facts: (i) effective contact between competent cells and transforming DNA molecules is established within a short time after mixing them together, and (ii) DNA molecules enter the competent B. subtilis cells in a linear fashion at a finite speed. From the number of DNA molecules initially attached to competent cells by brief exposure to transforming DNA in the first method or from the rate of DNA uptake by competent culture in the second method, the average number of DNA uptake sites is calculated to be 20 to 53 per competent cell.
摘要
提出了两种直接方法，用于通过测量感受态培养物对¹⁴C或³H标记的DNA摄取量，来估算枯草芽孢杆菌感受态细胞中脱氧核糖核酸（DNA）摄取位点的平均数量。利用了两个事实：（i）感受态细胞与转化DNA分子混合后短时间内即可建立有效接触，以及（ii）DNA分子以有限速度线性进入感受态枯草芽孢杆菌细胞。通过第一种方法中短暂暴露于转化DNA后最初附着在感受态细胞上的DNA分子数量，或通过第二种方法中感受态培养物的DNA摄取速率，计算出每个感受态细胞的DNA摄取位点平均数量为20至53个。
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