TAKAHASHI I
J Bacteriol. 1965 Feb;89(2):294-8. doi: 10.1128/jb.89.2.294-298.1965.
Takahashi, I. (McMaster University, Hamilton, Ontario, Canada). Transduction of sporogenesis in Bacillus subtilis. J. Bacteriol. 89:294-298. 1965.-A number of asporogenous mutants of Bacillus subtilis were isolated and genetic analyses with these mutants were carried out by means of transduction mediated by bacteriophage PBS 1. The ability to form spores could be restored by transduction in all mutants examined, with the exception of Sp(-)H12-3, although they were not transformable under my experimental conditions. The results of reciprocal transduction suggest that genetic loci at which a mutation can affect sporulation are numerous. A spore marker (sp 1) was transduced jointly with prototrophy and some antibiotic resistance markers (streptomycin, erythromycin, and neomycin). Other spore markers (sp N2-2, sp 170-2, and sp H12-4) were also linked to auxotrophic markers (ser, tyr, and phe) at a fairly high frequency. This linkage relationship indicates that genes controlling the formation of spores are located along the chromosome far apart from each other. The result also suggests that blocks in a variety of biochemical processes may result in the expression of the asporogenous phenotype.
高桥，I.（加拿大安大略省汉密尔顿市麦克马斯特大学）。枯草芽孢杆菌孢子形成的转导。《细菌学杂志》89:294 - 298。1965年。——分离出了一些枯草芽孢杆菌的无芽孢突变体，并通过噬菌体PBS 1介导的转导对这些突变体进行了遗传分析。在所检测的所有突变体中，除了Sp(-)H12 - 3之外，通过转导都可以恢复形成孢子的能力，尽管在我的实验条件下它们不能被转化。反向转导的结果表明，突变可影响孢子形成的基因位点很多。一个孢子标记（sp 1）与原养型以及一些抗生素抗性标记（链霉素、红霉素和新霉素）一起被转导。其他孢子标记（sp N2 - 2、sp 170 - 2和sp H12 - 4）也以相当高的频率与营养缺陷型标记（丝氨酸、酪氨酸和苯丙氨酸）连锁。这种连锁关系表明，控制孢子形成的基因在染色体上彼此相距很远。该结果还表明，各种生化过程中的阻断可能导致无芽孢表型的表达。
