米曲霉分生孢子中的海藻糖酶
Trehalase in conidia of Aspergillus oryzae.
作者信息
Horikoshi K, Ikeda Y
出版信息
J Bacteriol. 1966 May;91(5):1883-7. doi: 10.1128/jb.91.5.1883-1887.1966.
Abstract
Horikoshi, Koki (The Institute of Physical and Chemical Research, Bunkyo-ku, Tokyo, Japan), and Yonosuke Ikeda. Trehalase in conidia of Aspergillus oryzae. J. Bacteriol. 91:1883-1887. 1966.-Trehalases (soluble trehalase and coat-bound trehalase) were found in the conidia of Aspergillus oryzae, and the total activity of the trehalases increased during the germination process. The soluble trehalase was purified by diethylaminoethyl-cellulose column chromatography; its optimal pH, Michaelis constant, and heat stability were studied. In vitro, the trehalases were competitively inhibited by d-mannitol, which was also contained in the conidia. Since the trehalose content in the conidia decreased at an early stage of germination, it was assumed that trehalase might begin to hydrolyze trehalose after the inhibitory effect of d-mannitol decreased.
摘要
堀越浩史（日本东京文京区理化研究所）和池田米之助。米曲霉分生孢子中的海藻糖酶。《细菌学杂志》91:1883 - 1887。1966年。——在米曲霉的分生孢子中发现了海藻糖酶（可溶性海藻糖酶和附着于细胞壁的海藻糖酶），并且在萌发过程中海藻糖酶的总活性增加。通过二乙氨基乙基纤维素柱色谱法纯化了可溶性海藻糖酶；研究了其最适pH值、米氏常数和热稳定性。在体外，海藻糖酶受到d - 甘露醇的竞争性抑制，d - 甘露醇也存在于分生孢子中。由于分生孢子中的海藻糖含量在萌发早期下降，推测在d - 甘露醇的抑制作用减弱后，海藻糖酶可能开始水解海藻糖。
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