鸡干扰素的物理特性
PHYSICAL PROPERTIES OF CHICK INTERFERON.
作者信息
KREUZ L E, LEVY A H
出版信息
J Bacteriol. 1965 Feb;89(2):462-9. doi: 10.1128/jb.89.2.462-469.1965.
Kreuz, Leo E. (The Johns Hopkins University School of Medicine, Baltimore, Md.), and Allan H. Levy. Physical properties of chick interferon. J. Bacteriol. 89:462-469. 1965.-The sedimentation coefficient, diffusion coefficient, and molecular weight of chick-embryo interferon were determined by zone centrifugation and equilibrium sedimentation in cesium chloride density gradients, and by chromatography on Sephadex G-100 columns. Purified interferon is not available in quantities sufficient to permit direct analysis by chemical or physical means; its relative concentrations were determined, therefore, by bioassay. I(125)-human serum albumin was used as an internal reference in all experiments. The sedimentation coefficient of chick-embryo interferon is 2.2 to 2.3S; the diffusion coefficient is 9.5 x 10(-7) cm(2)sec(-1). A molecular weight of 26,000 was calculated from the sedimentation and diffusion coefficients, and a range of 25,000 to 34,000 daltons was obtained from equilibrium-sedimentation analyses.
克鲁兹，利奥·E.（约翰霍普金斯大学医学院，马里兰州巴尔的摩），以及艾伦·H.利维。鸡干扰素的物理性质。《细菌学杂志》89:462 - 469。1965年。 - 通过在氯化铯密度梯度中的区带离心和平衡沉降，以及在葡聚糖凝胶G - 100柱上进行色谱分析，测定了鸡胚干扰素的沉降系数、扩散系数和分子量。由于无法获得足够量的纯化干扰素以进行化学或物理方法的直接分析，因此通过生物测定法测定其相对浓度。在所有实验中，I(125)-人血清白蛋白用作内部参照。鸡胚干扰素的沉降系数为2.2至2.3S；扩散系数为9.5×10(-7) cm(2)sec(-1)。根据沉降和扩散系数计算出分子量为26,000，通过平衡沉降分析得到的分子量范围为25,000至34,000道尔顿。
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