Saunders G F, Campbell L L
J Bacteriol. 1966 Jan;91(1):340-8. doi: 10.1128/jb.91.1.340-348.1966.
Saunders, Grady F. (University of Illinois, Urbana), and L. Leon Campbell. Characterization of a thermophilic bacteriophage for Bacillus stearothermophilus. J. Bacteriol. 91:340-348. 1965.-The biological and physical-chemical properties of the thermophilic bacteriophage TP-84 were investigated. TP-84 was shown to be lytic for 3 of 24 strains of Bacillus stearothermophilus tested over the temperature range of 43 to 76 C. The latent period of TP-84 on B. stearothermophilus strain 10 was 22 to 24 min. TP-84 has a hexagonal head, 53 mmu in diameter and 30 mmu on a side; its tail is 130 mmu long and 3 to 5 mmu wide. The phage has an S(50) (20,w) of 436, and bands at a density of 1.508 g/cc in CsCl (pH 8.5). The diffusion coefficient of TP-84 was calculated to be 6.19 x 10(-8) cm(2)/sec. From the sedimentation and diffusion data, a particle molecular weight of 50 million daltons was calculated for TP-84. The phage DNA has a base composition of 42% guanine + cytosine, deduced from buoyant density and melting temperature measurements.
桑德斯，格雷迪·F.（伊利诺伊大学厄巴纳分校）和L.利昂·坎贝尔。嗜热脂肪芽孢杆菌嗜热噬菌体的特性。《细菌学杂志》91:340 - 348。1965年。——研究了嗜热噬菌体TP - 84的生物学和物理化学特性。结果表明，在43至76摄氏度的温度范围内，TP - 84能裂解所测试的24株嗜热脂肪芽孢杆菌中的3株。TP - 84在嗜热脂肪芽孢杆菌10菌株上的潜伏期为22至24分钟。TP - 84有一个六边形头部，直径53纳米，边长30纳米；其尾部长130纳米，宽3至5纳米。该噬菌体的S(50)（20，w）为436，在CsCl（pH 8.5）中的密度带为1.508克/立方厘米。计算得出TP - 84的扩散系数为6.19×10(-8)平方厘米/秒。根据沉降和扩散数据，计算出TP - 84的颗粒分子量为5000万道尔顿。从浮力密度和熔解温度测量推断，该噬菌体DNA的碱基组成为42%鸟嘌呤 + 胞嘧啶。
