鼻病毒和脊髓灰质炎病毒的生物物理与生化研究。II. 鼻病毒颗粒的化学与流体动力学分析。
Biophysical and biochemical studies on rhinovirus and poliovirus. II. Chemical and hydrodynamic analysis of the rhinovirion.
作者信息
McGregor S, Mayor H D
出版信息
J Virol. 1971 Jan;7(1):41-6. doi: 10.1128/JVI.7.1.41-46.1971.
Abstract
Chemical analysis of rhinovirus 14 revealed a ribonucleic acid (RNA) content of 29.8% and a high adenylic acid content (35%). A partial specific volume of 0.682 cm(3)/g was obtained for the rhinovirion. Rhinovirus and poliovirus had identical sedimentation coefficients of 158S. A diffusion coefficient of 1.71 x 10(-7) cm(2)/sec was consistent with a hydrated diameter of 25 nm for the rhinovirion. The calculated molecular weights of the rhinovirion and its genome were 7.1 x 10(6) and 2.1 x 10(6) daltons, respectively. Sedimentation analysis of infectious RNA confirmed the similarity of the molecular size of the poliovirus and rhinovirus genomes.
摘要
对鼻病毒14的化学分析显示，其核糖核酸（RNA）含量为29.8%，腺苷酸含量较高（35%）。鼻病毒颗粒的偏比容为0.682 cm³/g。鼻病毒和脊髓灰质炎病毒的沉降系数相同，均为158S。扩散系数为1.71×10⁻⁷ cm²/秒，这与鼻病毒颗粒25纳米的水化直径相符。计算得出鼻病毒颗粒及其基因组的分子量分别为7.1×10⁶和2.1×10⁶道尔顿。感染性RNA的沉降分析证实了脊髓灰质炎病毒和鼻病毒基因组分子大小的相似性。
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