肾细胞癌中细胞DNA含量的生物学及临床意义

Biological and clinical implication of cellular DNA content in renal cell carcinomas.

作者信息

Di Silverio F, Gallucci M, Flammia G P, De Vico A, Caponera M, Eleuteri P, Forte D, Cavallo D, De Vita R

机构信息

Department of Urology, University of Rome La Sapienza, Italy.

出版信息

Eur Urol. 1992;21 Suppl 1:43-7. doi: 10.1159/000474888.

DOI:10.1159/000474888

PMID:1425835

Abstract

DNA flow cytometric analysis (FCM) was performed on surgical bioptic samples taken from 82 renal cell carcinomas. FCM has evidenced that 35% (29/82) of renal carcinomas resulted diploid, 65% (53/82) aneuploid and of the latter 22% (12/53) multiclonal. Our results do not indicate any relationship among cytometric ploidy, Fuhrman grading, Robson and pTNM staging. A possible interesting increase of aneuploidy frequency was observed between the NMV (66%) subgroup and the no zero NMV (90%) subgroup, while in diploid patients these values were 40% and 10%, respectively. Follow-up data evidence a significant difference in survival pattern of patients between diploid and aneuploid groups. In conclusion, our results show that cytometric ploidy is a potential important prognostic parameter in survival term.

摘要

对取自82例肾细胞癌的手术活检样本进行了DNA流式细胞术分析（FCM）。FCM已证明，35%（29/82）的肾癌为二倍体，65%（53/82）为非整倍体，其中22%（12/53）为多克隆。我们的结果未表明细胞计量倍体、福尔曼分级、罗布森分期和pTNM分期之间存在任何关系。在NMV（66%）亚组和非零NMV（90%）亚组之间观察到非整倍体频率可能存在有趣的增加，而在二倍体患者中，这些值分别为40%和10%。随访数据表明二倍体组和非整倍体组患者的生存模式存在显著差异。总之，我们的结果表明，细胞计量倍体是生存期潜在的重要预后参数。