Martin R E, Bazan N G
LSU Eye Center and Neuroscience Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112-2234.
Exp Eye Res. 1992 Aug;55(2):307-14. doi: 10.1016/0014-4835(92)90195-x.
We used dual-wavelength fluorescence microscopy and monoclonal antibodies to growth-associated protein (GAP-43) and nerve cell adhesion molecule (N-CAM) to identify these proteins in nerve fibers of normal rat and rabbit corneas. Overlapping immunoreactivity of GAP-43 and N-CAM was evident along nerve fibers of rabbit corneal sections, suggesting that GAP-43 is constitutively expressed in these sensory nerves. The immune reaction of monoclonal antibody to GAP-43 and [125I]protein A was used to quantitate relative amounts of GAP-43 in the normal cornea and in a cornea subjected to a de-epithelializing wound. Collectively these findings imply that GAP-43 is axoplasmically transported from cells in the trigeminal (or superior cervical) ganglion to the cornea. Moreover, these data indicate that GAP-43 appears to be involved in the remodeling of corneal nerves that is necessary for normal innervation.
我们使用双波长荧光显微镜以及针对生长相关蛋白（GAP - 43）和神经细胞黏附分子（N - CAM）的单克隆抗体，来鉴定正常大鼠和兔角膜神经纤维中的这些蛋白质。在兔角膜切片的神经纤维上，GAP - 43和N - CAM的免疫反应重叠现象明显，这表明GAP - 43在这些感觉神经中持续表达。利用针对GAP - 43的单克隆抗体与[125I]蛋白A的免疫反应，对正常角膜和去上皮化伤口处理后的角膜中GAP - 43的相对含量进行定量。这些发现共同表明，GAP - 43是从三叉神经（或颈上神经节）的细胞通过轴浆运输到角膜的。此外，这些数据表明，GAP - 43似乎参与了正常神经支配所必需的角膜神经重塑过程。
