嘌呤霉素：对大肠杆菌15T中信使核糖核酸合成及β-半乳糖苷酶形成的影响

PUROMYCIN: EFFECT ON MESSENGER RNA SYNTHESIS AND BETA-GALACTOSIDASE FORMATION IN ESCHERICHIA COLI 15T.

作者信息

SELLS B H

出版信息

Science. 1965 Apr 16;148(3668):371-3. doi: 10.1126/science.148.3668.371.

DOI:10.1126/science.148.3668.371

Abstract

Incubation of Escherichia coli 15T(-) in the presence of puromycin inhibits the inducible formation of beta-galactosidase to a greater extent than it inhibits protein synthesis. In E. coli 33.00 beta-galactosidase formation is also more sensitive to the presence of puromycin than is protein synthesis. In the presence of glycerol (but not in its absence) puromycin prevents the production of messenger RNA for beta-galactosidase, presumably as a result of catabolite repression.

摘要

在嘌呤霉素存在的情况下培养大肠杆菌15T(-)，对β-半乳糖苷酶的诱导形成的抑制作用比对蛋白质合成的抑制作用更大。在大肠杆菌33.00中，β-半乳糖苷酶的形成对嘌呤霉素的存在也比蛋白质合成更敏感。在甘油存在的情况下（但在其不存在时则不然），嘌呤霉素可阻止β-半乳糖苷酶信使RNA的产生，这可能是分解代谢物阻遏的结果。