Nilsson P, Mannermaa R M, Oikarinen J, Grundström T
Department of Applied Cell and Molecular Biology, University of Umeå, Sweden.
FEBS Lett. 1992 Nov 16;313(1):67-70. doi: 10.1016/0014-5793(92)81186-p.
Histone H1 acts as a general repressor of transcription in eukaryotes by organizing nucleosomes into inaccessible condensed forms of chromatin. The capability of H1 to bind to DNA with some sequence specificity is likely to be critical in the control of these processes. We show here that ATP and several other nucleotides, including non-hydrolyzable derivatives, can inhibit DNA binding of H1. The results also show that ATP differentially affects binding of H1 to DNA in a fashion enhancing nucleotide sequence specificity of the binding. The study suggests a novel mechanism of modulation of H1 activity that has important implications for the role of H1 as a transcriptional regulator.
组蛋白H1通过将核小体组织成难以接近的凝聚染色质形式，在真核生物中作为转录的一般阻遏物发挥作用。H1以一定的序列特异性与DNA结合的能力，可能在这些过程的控制中至关重要。我们在此表明，ATP和其他几种核苷酸，包括不可水解的衍生物，可抑制H1与DNA的结合。结果还表明，ATP以增强结合的核苷酸序列特异性的方式，差异性地影响H1与DNA的结合。该研究提示了一种调节H1活性的新机制，这对H1作为转录调节因子的作用具有重要意义。
