Loy R A, Loukides J A, Polan M L
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut.
Fertil Steril. 1992 Oct;58(4):733-9. doi: 10.1016/s0015-0282(16)55320-5.
To determine whether tumor necrosis factor alpha (TNF-alpha) messenger ribonucleic acid (mRNA) levels in human peripheral monocytes are regulated by ovarian steroids.
Human granulosa-luteal cells and cultured, activated human peripheral monocytes were subjected to Northern blot analysis for TNF-alpha mRNA.
Academic research laboratory.
Two human female volunteers of reproductive age and in the luteal phase of the menstrual cycle.
Human granulosa-luteal cells produce TNF-alpha mRNA. Physiological levels of progesterone (P) and estradiol (E2) modulate TNF-alpha mRNA from peripheral blood monocytes with an apparent inverse relationship between steroid concentration and TNF-alpha message.
Progesterone and E2 at physiological concentrations regulate TNF-alpha mRNA production. The P antagonist mifepristone (RU486) and the E2 agonist/antagonist tamoxifen modulate total TNF-alpha mRNA levels, suggesting involvement of specific receptors.
确定人外周血单核细胞中肿瘤坏死因子α（TNF-α）信使核糖核酸（mRNA）水平是否受卵巢甾体激素调节。
对人颗粒黄体细胞和培养的、活化的人外周血单核细胞进行TNF-α mRNA的Northern印迹分析。
学术研究实验室。
两名处于月经周期黄体期的育龄女性志愿者。
人颗粒黄体细胞产生TNF-α mRNA。孕酮（P）和雌二醇（E2）的生理水平调节外周血单核细胞中的TNF-α mRNA，甾体激素浓度与TNF-α信息之间存在明显的负相关关系。
生理浓度的孕酮和E2调节TNF-α mRNA的产生。P拮抗剂米非司酮（RU486）和E2激动剂/拮抗剂他莫昔芬调节总TNF-α mRNA水平，提示特定受体参与其中。
