Lee B Y, Huynh T, Prichard L E, McGuire J, Polan M L
Department of Gynecology and Obstetrics, Stanford University School of Medicine, CA 94305, USA.
Biochem Biophys Res Commun. 1995 Apr 6;209(1):279-85. doi: 10.1006/bbrc.1995.1500.
This study investigated whether gonadal steroids modulate the expression of the cytokine Interleukin-1 receptor antagonist in monocytes. Human male peripheral monocytes were isolated and cultured in serum free media with serially diluted concentrations of estradiol and progesterone. mRNA expressions with increasing steroid concentrations were compared by reverse transcription-polymerase chain reaction for intracellular and secretory interleukin-1 receptor antagonist specific primers and glyceraldehyde 3-phosphate dehydrogenase primers. Monocyte expression of secretory Interleukin-1 receptor antagonist mRNA was significantly elevated in the presence of normal physiological levels of estradiol (10(-11) M) and progesterone (10(-8) M), while expression was suppressed by higher concentrations of steroids. Intracellular receptor antagonist was also detected. This study is the first to describe the dose related response of cytokine interleukin-1 receptor antagonist to gonadal steroids.
本研究调查了性腺类固醇是否调节单核细胞中细胞因子白细胞介素-1受体拮抗剂的表达。分离人类男性外周血单核细胞，并在含有系列稀释浓度雌二醇和孕酮的无血清培养基中培养。通过逆转录-聚合酶链反应，针对细胞内和分泌型白细胞介素-1受体拮抗剂特异性引物以及甘油醛-3-磷酸脱氢酶引物，比较随着类固醇浓度增加时的mRNA表达。在正常生理水平的雌二醇（10(-11) M）和孕酮（10(-8) M）存在的情况下，分泌型白细胞介素-1受体拮抗剂mRNA的单核细胞表达显著升高，而较高浓度的类固醇则抑制其表达。还检测到了细胞内受体拮抗剂。本研究首次描述了细胞因子白细胞介素-1受体拮抗剂对性腺类固醇的剂量相关反应。
