CHRISTIAN C L, DESIMONE A R, ABRUZZO J L
J Exp Med. 1965 Feb 1;121(2):309-21. doi: 10.1084/jem.121.2.309.
Complement-fixing anti-DNA antibodies were detected in a minority of sera of rabbits hyperimmunized with killed Gram-negative bacteria. The C'-fixing property of DNA was lost after DNase treatment. Preferential reactivity with denatured DNA was observed. The antisera reacted with DNA preparations derived from rabbit bone marrow and thymus, calf thymus, pneumococci, salmon sperm, and Escherichia coli. E. coli DNA was less effective than preparations of mammalian and salmon sperm DNA in fixation of C'. Inhibition of DNA C' fixation by nucleotides and nucleosides was observed. The bulk of anti-DNA activity was associated with the low molecular weight antibody fraction.
在经灭活革兰氏阴性菌超免疫的少数兔血清中检测到补体结合抗DNA抗体。DNA经脱氧核糖核酸酶处理后丧失了补体结合特性。观察到与变性DNA有优先反应性。抗血清与源自兔骨髓和胸腺、小牛胸腺、肺炎球菌、鲑鱼精子和大肠杆菌的DNA制剂发生反应。在补体结合方面，大肠杆菌DNA不如哺乳动物和鲑鱼精子DNA制剂有效。观察到核苷酸和核苷对DNA补体结合有抑制作用。大部分抗DNA活性与低分子量抗体部分有关。
