Ibrahim A, Liesack W, Pike S, Stackebrandt E
Department of Microbiology, University of Queensland, Australia.
FEMS Microbiol Lett. 1992 Oct 1;76(1-2):63-6. doi: 10.1016/0378-1097(92)90364-t.
A primer set designed to amplify the enterotoxin (yst) gene of pathogenic Yersinia enterocolitica strains generated two different electrophoretic profiles of the target sequence when a collection of strains of worldwide origin was screened. Serovars O:1,3; O:2a,3; O:3; O:5,27 and O:9, known as European strains, produced a 200-bp fragment that matched the size of the target sequence. However, serovars O:4,32; O:8; O:13a,13b; O:20 and O:21, known as American strains, generated two fragments of 1.4 and 1.6 kb. The amplified products of one American strain were sequenced and the presence of the yst gene was confirmed in both fragments. Thus, the potential of the polymerase chain reaction to be used as an epidemiological tool in differentiation between the two clusters of pathogenic strains of Y. enterocolitica could be demonstrated.
当对一组来自世界各地的致病性小肠结肠炎耶尔森氏菌菌株进行筛选时，用于扩增该菌肠毒素（yst）基因的引物组产生了两种不同的目标序列电泳图谱。血清型O:1,3；O:2a,3；O:3；O:5,27和O:9，即所谓的欧洲菌株，产生了一个与目标序列大小相符的200碱基对片段。然而，血清型O:4,32；O:8；O:13a,13b；O:20和O:21，即所谓的美国菌株，产生了1.4和1.6千碱基对的两个片段。对一株美国菌株的扩增产物进行了测序，并在两个片段中均证实了yst基因的存在。因此，可以证明聚合酶链反应在区分小肠结肠炎耶尔森氏菌两群致病菌株方面作为一种流行病学工具的潜力。
