Weynants V, Jadot V, Denoel P A, Tibor A, Letesson J J
Unité d'Immunologie-Microbiologie, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
J Clin Microbiol. 1996 May;34(5):1224-7. doi: 10.1128/jcm.34.5.1224-1227.1996.
Yersinia enterocolitica is the etiologic agent of a range of clinical situations in humans, but only a small number of serotypes are involved. Among these, Y. enterocolitica O:3 is the most frequently implicated. A PCR method was developed to detect Y. enterocolitica O:3. For this purpose, two pairs of primers were designed to amplify two fragments of the rfb cluster of Y. enterocolitica O:3: a 253-bp fragment of the rfbB gene and a 405-bp fragment of the rfbC gene. A specific detection was obtained only with rfbC primers, which yielded a PCR product of the expected size exclusively with pathogenic Y. enterocolitica of serotype O:3. This pair of primers was combined with the ail, inv, and virF primers previously described (H. Nakajima, M. Inoue, T. Mori, K.-I. Itoh, E. Arakawa, and H. Watanabe, J. Clin. Microbiol. 30:2484-2486, 1992) to allow both the detection and the differentiation between Y. pseudotuberculosis, pathogenic Y. enterocolitica of serotype O:3 and other pathogenic Y. enterocolitica.
小肠结肠炎耶尔森菌是人类一系列临床病症的病原体，但只有少数血清型与之相关。其中，小肠结肠炎耶尔森菌O:3是最常涉及的血清型。开发了一种用于检测小肠结肠炎耶尔森菌O:3的PCR方法。为此，设计了两对引物来扩增小肠结肠炎耶尔森菌O:3的rfb基因簇的两个片段：rfbB基因的一个253 bp片段和rfbC基因的一个405 bp片段。仅用rfbC引物获得了特异性检测结果，该引物仅与O:3血清型的致病性小肠结肠炎耶尔森菌产生预期大小的PCR产物。将这对引物与先前描述的ail、inv和virF引物（H. Nakajima、M. Inoue、T. Mori、K.-I. Itoh、E. Arakawa和H. Watanabe，《临床微生物学杂志》30:2484 - 2486，1992年）结合使用，以实现对假结核耶尔森菌、O:3血清型致病性小肠结肠炎耶尔森菌和其他致病性小肠结肠炎耶尔森菌的检测和鉴别。
