热对牛痘病毒的感染、抗体吸收及干扰能力的影响
EFFECTS OF HEAT ON THE INFECTING, ANTIBODY-ABSORBING, AND INTERFERING POWERS OF VACCINIA VIRUS.
作者信息
GALASSO G J, SHARP D G
出版信息
J Bacteriol. 1965 Mar;89(3):611-6. doi: 10.1128/jb.89.3.611-616.1965.
Galasso, G. J. (University of North Carolina School of Medicine, Chapel Hill), and D. G. Sharp. Effects of heat on the infecting, antibody-absorbing, and interfering powers of vaccinia virus. J. Bacteriol. 89:611-616. 1965.-At 56 C the infectivity of vaccinia virus particles is destroyed rapidly, but even when it is reduced by a factor of 10(6) the particles are capable of producing strong interference in L cells. The rate constant K for thermal inactivation of plaque-forming power is greater than that for interfering power by the factor e(3.8) or about 45 times. At 37 C both properties of the virus decline more slowly and at equal rates. The temperature coefficient of K is discontinuous in the region of 40 C, indicating quite different activation energies for the reactions above and below this critical point. The degradation of interfering power exhibits a similar discontinuity, although less in magnitude, but none has been found in the antibody-reactive power of the virus, which is much more heat resistant.
加拉索，G. J.（北卡罗来纳大学医学院，教堂山分校），以及D. G. 夏普。热对痘苗病毒感染性、抗体吸收能力及干扰能力的影响。《细菌学杂志》89:611 - 616。1965年。——在56℃时，痘苗病毒颗粒的感染性迅速被破坏，但即便其降低了10⁶倍，这些颗粒仍能够在L细胞中产生强烈干扰。形成噬菌斑能力的热失活速率常数K比干扰能力的速率常数大e³·⁸倍，即约45倍。在37℃时，病毒的这两种特性下降得更慢且速率相同。K的温度系数在40℃区域是不连续的，表明在该临界点之上和之下的反应具有完全不同的活化能。干扰能力的降解也表现出类似的不连续性，尽管幅度较小，但在病毒的抗体反应能力中未发现不连续性，其对热的耐受性要强得多。
Zotero 插件