Galasso G J, Sharp D G
J Bacteriol. 1965 Oct;90(4):1138-42. doi: 10.1128/jb.90.4.1138-1142.1965.
Galasso, G. J. (University of North Carolina School of Medicine, Chapel Hill), and D. G. Sharp. Effect of particle aggregation on the survival of irradiated vaccinia virus. J. Bacteriol. 90:1138-1142. 1965.-The survival curve (plaque titer versus time) of vaccinia virus irradiated with ultraviolet light was found to be strongly dependent, in both shape and general slope, on the degree of aggregation among the virus particles. When special care was taken to produce completely dispersed virus, a straight steep line resulted. Some severely aggregated preparations produced a straight line also, but of much less slope. Thus, the straight line, often taken as evidence of complete dispersion, is not a reliable measure for vaccinia virus. None of the experimental curves resembled the classical multihit curve predicted by the Poisson function for the behavior of uniformly aggregated virus. The aggregation was observed, by electron microscopy, to be nonuniform, and of such a distribution that the survival curves can be qualitatively accounted for in terms of multiplicity reactivation of irradiated virus within cells that receive groups of two or more particles. The greatly increased survival value of aggregates of virus, which indicates multiplicity reactivation, is worthy of note.
加拉索，G. J.（北卡罗来纳大学医学院，教堂山分校），以及D. G. 夏普。颗粒聚集对经辐照痘苗病毒存活的影响。《细菌学杂志》90:1138 - 1142。1965年。——发现经紫外线辐照的痘苗病毒的存活曲线（噬斑滴度与时间的关系）在形状和总体斜率上都强烈依赖于病毒颗粒之间的聚集程度。当特别小心地制备完全分散的病毒时，会得到一条陡峭的直线。一些严重聚集的制剂也产生了一条直线，但斜率小得多。因此，通常被视为完全分散证据的直线，对于痘苗病毒来说并不是一个可靠的衡量标准。没有一条实验曲线类似于泊松函数预测的均匀聚集病毒行为的经典多击曲线。通过电子显微镜观察到聚集是不均匀的，并且其分布使得存活曲线可以根据接受两个或更多颗粒组的细胞内辐照病毒的多重复活在定性上得到解释。病毒聚集体存活值的大幅增加，表明存在多重复活，这一点值得注意。
