ARM H G, FLOYD T M, FABER J E, HAYES J R
J Bacteriol. 1965 Mar;89(3):803-9. doi: 10.1128/jb.89.3.803-809.1965.
Arm, H. G. (Naval Medical Research Institute, Bethesda, Md.), T. M. Floyd, J. E. Faber, and J. R. Hayes. Use of ligated segments of rabbit small intestine in experimental shigellosis. J. Bacteriol. 89:803-809. 1965.-Inoculation of ligated segments of small intestine in living rabbits with broth cultures or resting-cell suspensions of recently isolated strains of Shigella caused distension of the segments by accumulation of exudate within 12 hr. Histological changes characteristic of an inflammatory response were similar to those of human bacillary dysentery. Tissue response and accumulation of exudate preceded demonstrable increase in numbers of shigellae inoculated as 2 x 10(10) resting cells. Capability of shigellae to provoke intestinal response was not related to any particular serological group. The active principles concerned with eliciting intestinal response were associated only with preparations containing living organisms. Ability of recently isolated strains to elicit response diminished rapidly during culture on artificial media. The capability was preserved indefinitely by lyophilization soon after isolation from acute bacillary dysentery infections of man. Advantages of using shigellae recently isolated for investigating possible mechanisms of pathogenesis were indicated. During the summer months, the rabbit small intestine was refractory to the activity of shigellae, and positive responses were not observed. Use of ligated segments of rabbit small intestine qualified as an indicator of virulence for the rabbit; and, virulence for the rabbit showed a high degree of correlation with a short period of culture of shigellae on artificial media after isolation from human bacillary dysentery infections.
阿姆，H.G.（海军医学研究所，马里兰州贝塞斯达），T.M.弗洛伊德，J.E.法伯，以及J.R.海斯。兔小肠结扎段在实验性志贺菌病中的应用。《细菌学杂志》89:803 - 809。1965年。——用肉汤培养物或最近分离的志贺菌菌株的静止细胞悬液接种活兔的小肠结扎段，12小时内渗出物积聚导致肠段扩张。炎症反应的组织学变化特征与人细菌性痢疾相似。在接种2×10¹⁰个静止细胞时，组织反应和渗出物积聚先于接种的志贺菌数量明显增加。志贺菌引发肠道反应的能力与任何特定血清群无关。引发肠道反应的活性成分仅与含有活生物体的制剂有关。最近分离的菌株在人工培养基上培养时引发反应的能力迅速下降。从人类急性细菌性痢疾感染中分离后不久通过冻干可无限期保存该能力。指出了使用最近分离的志贺菌来研究发病机制可能机制的优势。在夏季，兔小肠对志贺菌的活性不敏感，未观察到阳性反应。兔小肠结扎段的使用可作为兔毒力的指标；并且，兔毒力与从人类细菌性痢疾感染中分离后在人工培养基上短期培养志贺菌显示出高度相关性。
