WHEELER W C, HANKS J H
J Bacteriol. 1965 Mar;89(3):889-96. doi: 10.1128/jb.89.3.889-896.1965.
Wheeler, William C. (Johns Hopkins University-Leonard Wood Memorial Leprosy Research Laboratory, Baltimore, Md.), and John H. Hanks. Utilization of external growth factors by intracellular microbes: Mycobacterium paratuberculosis and wood pigeon mycobacteria. J. Bacteriol. 89:889-896. 1965.-The extent to which the intracellular growth of microbes is dependent upon capacities for growth in vitro has been investigated by use of organisms which require a specific factor, mycobactin, for isolation and growth on conventional mycobacterial media. Similarities between growth on bacteriological media and within tissue cells have been demonstrated by examining the responses of Mycobacterium paratuberculosis and wood pigeon mycobacteria to mycobactin, iron, CO(2), and glycerol, and to the low pH which characterizes phagocytic vacuoles. The results indicate that success of intracellular infections depends upon the independent growth of microbes and that the phagocytic vacuoles of sheep and mouse macrophages seem to be freely accessible to bacterial growth factors, minerals, and substrates. Because these factors did not modify the appearance, overall metabolism, or surface properties of macrophages, it was concluded that the intracellular growth of microbes is not determined solely by the components, metabolism, or immunological properties of host cells, but is influenced to an important degree by compounds and conditions provided by extracellular environments.
惠勒，威廉·C.（约翰斯·霍普金斯大学-伦纳德·伍德纪念麻风病研究实验室，马里兰州巴尔的摩），以及约翰·H.汉克斯。细胞内微生物对外部生长因子的利用：副结核分枝杆菌和林鸽分枝杆菌。《细菌学杂志》89:889 - 896。1965年。- 通过使用在传统分枝杆菌培养基上分离和生长需要特定因子（分枝杆菌素）的生物体，研究了微生物的细胞内生长在多大程度上依赖于体外生长能力。通过检测副结核分枝杆菌和林鸽分枝杆菌对分枝杆菌素、铁、二氧化碳、甘油以及吞噬泡特征性低pH值的反应，证明了在细菌学培养基上的生长与在组织细胞内的生长之间的相似性。结果表明，细胞内感染的成功取决于微生物的独立生长，并且绵羊和小鼠巨噬细胞的吞噬泡似乎可自由获取细菌生长因子、矿物质和底物。由于这些因子并未改变巨噬细胞的外观、整体代谢或表面特性，得出的结论是，微生物的细胞内生长并非仅由宿主细胞的成分、代谢或免疫特性决定，而是在很大程度上受细胞外环境提供的化合物和条件影响。
