MORRISON N E
J Bacteriol. 1965 Mar;89(3):762-7. doi: 10.1128/jb.89.3.762-767.1965.
Morrison, Norman E. (Johns Hopkins University-Leonard Wood Memorial Leprosy Research Laboratory, Baltimore, Md.). Circumvention of the mycobactin requirement of Mycobacterium paratuberculosis. J. Bacteriol. 89:762-767. 1965.-The mycobactin growth requirement of Mycobacterium paratuberculosis was circumvented on glucose-containing synthetic medium with an initial pH of 5.5. Mycobactin was required during the first transfer on the synthetic medium. Subsequent transfers have grown in the absence of mycobactin. The growth of mycobactin-"independent" strains of M. paratuberculosis on the synthetic medium was found to be stimulated by low concentrations of mycobactin. The circumvention of the mycobactin requirement appears to depend upon the properties of the medium and not upon having created conditions which promote endogenous mycobactin synthesis. Investigation of the glucose-containing synthetic medium showed that: (i) growth stimulatory compounds were formed during autoclaving, and (ii) compared with neutrality a pH of 5.5 gave markedly increased pellicle yields. It was suggested that the growth-stimulatory compounds formed during autoclaving may in part be responsible for the circumvention of the mycobactin requirement.
莫里森，诺曼·E.（约翰霍普金斯大学 - 伦纳德·伍德纪念麻风病研究实验室，马里兰州巴尔的摩）。副结核分枝杆菌对分枝杆菌素需求的规避。《细菌学杂志》89:762 - 767。1965年。——在初始pH值为5.5的含葡萄糖合成培养基上，副结核分枝杆菌对分枝杆菌素的生长需求被规避。在合成培养基上首次传代时需要分枝杆菌素。后续传代在无分枝杆菌素的情况下生长。发现低浓度的分枝杆菌素能刺激副结核分枝杆菌“不依赖分枝杆菌素”菌株在合成培养基上的生长。对分枝杆菌素需求的规避似乎取决于培养基的特性，而非创造了促进内源性分枝杆菌素合成的条件。对含葡萄糖合成培养基的研究表明：（i）高压灭菌过程中形成了生长刺激化合物，（ii）与中性pH相比，pH值为5.5时菌膜产量显著增加。有人提出，高压灭菌过程中形成的生长刺激化合物可能部分导致了对分枝杆菌素需求的规避。
