大肠杆菌细胞组成的环境控制
ENVIRONMENTAL CONTROL OF CELL COMPOSITION IN ESCHERICHIA COLI.
作者信息
WRIGHT D N, LOCKHART W R
出版信息
J Bacteriol. 1965 Apr;89(4):1026-31. doi: 10.1128/jb.89.4.1026-1031.1965.
Wright, D. N. (Iowa State University, Ames), and W. R. Lockhart. Environmental control of cell composition in Escherichia coli. J. Bacteriol. 89:1026-1031. 1965.-Escherichia coli was grown in continuous culture at various rates in a defined medium with either the carbon or the nitrogen source as rate-limiting substrate. Average cell size and the cellular content of ribonucleic acid, protein, and free amino acids varied both with growth rate and with the identity of the limiting nutrient, being greatest at high growth rates, and greater for nitrogen-limited than for carbon-limited populations. Immunoelectrophoresis of cell extracts revealed antigenic components associated uniquely with growth rate, and others associated with the identity of the limiting nutrient on which cells had been grown. Cell composition thus appears to reflect the physiological adjustment of a microbial population to its total environment.
赖特，D. N.（衣阿华州立大学，艾姆斯），以及W. R. 洛克哈特。大肠杆菌细胞组成的环境控制。《细菌学杂志》89:1026 - 1031。1965年。——大肠杆菌在限定培养基中以不同速率进行连续培养，以碳源或氮源作为限速底物。平均细胞大小以及核糖核酸、蛋白质和游离氨基酸的细胞含量随生长速率和限制营养物的种类而变化，在高生长速率时最大，并且氮限制群体比碳限制群体更大。细胞提取物的免疫电泳显示出与生长速率独特相关的抗原成分，以及与细胞生长所依赖的限制营养物种类相关的其他成分。因此，细胞组成似乎反映了微生物群体对其整体环境的生理调节。
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