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同步分裂的细菌培养物。I. 大肠杆菌中必需氨基酸耗尽和重新补充后的同步性。

Synchronously dividing bacterial cultures. I. Synchrony following depletion and resupplementation of a required amino acid in Escerichia coli.

作者信息

Matney T S, Suit J C

出版信息

J Bacteriol. 1966 Oct;92(4):960-6. doi: 10.1128/jb.92.4.960-966.1966.

DOI:10.1128/jb.92.4.960-966.1966
PMID:5332875
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC276361/
Abstract

Matney, Thomas S. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas), and Joan C. Suit. Synchronously dividing bacterial cultures. I. Synchrony following depletion and resupplementation of a required amino acid in Escherichia coli. J. Bacteriol. 92:960-966. 1966.-A procedure was developed for phasing large-volume cultures of Escherichia coli K-12 with regard to cell division. The method consists of permitting the bacteria to exhaust a growth-limiting supply of a required amino acid, starving the culture, resupplementing with an excess of the amino acid, and following the ensuing growth by usual counting procedures.

摘要

马特尼,托马斯·S.(得克萨斯大学MD安德森医院和肿瘤研究所,得克萨斯州休斯顿),以及琼·C.休伊特。同步分裂的细菌培养物。一、大肠杆菌中必需氨基酸耗尽和重新补充后的同步化。《细菌学杂志》92:960 - 966。1966年。——开发了一种使大肠杆菌K - 12大量培养物在细胞分裂方面同步化的方法。该方法包括让细菌耗尽所需氨基酸的生长限制供应,使培养物饥饿,用过量的氨基酸重新补充,然后通过常规计数程序跟踪随后的生长情况。

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Synchronously dividing bacterial cultures. I. Synchrony following depletion and resupplementation of a required amino acid in Escerichia coli.同步分裂的细菌培养物。I. 大肠杆菌中必需氨基酸耗尽和重新补充后的同步性。
J Bacteriol. 1966 Oct;92(4):960-6. doi: 10.1128/jb.92.4.960-966.1966.
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引用本文的文献

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本文引用的文献

1
A RELATION OF NUCLEIC ACID SYNTHESIS TO RADIATION-INDUCED MUTATION FREQUENCY IN BACTERIA.细菌中核酸合成与辐射诱导突变频率的关系
Proc Natl Acad Sci U S A. 1957 Oct 15;43(10):871-83. doi: 10.1073/pnas.43.10.871.
2
The nucleic acids of plant tissues; the extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid.植物组织的核酸;脱氧戊糖核酸和戊糖核酸的提取与测定
Arch Biochem. 1950 Feb;25(2):262-76.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
A genetic locus for the regulation of ribonucleic acid synthesis.一个用于调节核糖核酸合成的基因位点。
Proc Natl Acad Sci U S A. 1961 Dec 15;47(12):2005-14. doi: 10.1073/pnas.47.12.2005.
5
Thymine deficiency and the normal DNA replication cycle. I.胸腺嘧啶缺乏与正常DNA复制周期。I.
J Mol Biol. 1961 Apr;3:144-55. doi: 10.1016/s0022-2836(61)80041-7.
6
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
7
Thymine incorporation and metabolism by various classes of thymine-less bacteria.各类胸腺嘧啶营养缺陷型细菌对胸腺嘧啶的掺入与代谢
J Gen Microbiol. 1965 Dec;41(3):321-33. doi: 10.1099/00221287-41-3-321.
8
A preliminary map of genomic sites for F-attachment in Escherichia coli K12.大肠杆菌K12中F因子附着的基因组位点初步图谱。
Biochem Biophys Res Commun. 1964 Oct 14;17(3):278-81. doi: 10.1016/0006-291x(64)90397-3.