同步分裂的细菌培养物。I. 大肠杆菌中必需氨基酸耗尽和重新补充后的同步性。
Synchronously dividing bacterial cultures. I. Synchrony following depletion and resupplementation of a required amino acid in Escerichia coli.
作者信息
Matney T S, Suit J C
出版信息
J Bacteriol. 1966 Oct;92(4):960-6. doi: 10.1128/jb.92.4.960-966.1966.
Abstract
Matney, Thomas S. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas), and Joan C. Suit. Synchronously dividing bacterial cultures. I. Synchrony following depletion and resupplementation of a required amino acid in Escherichia coli. J. Bacteriol. 92:960-966. 1966.-A procedure was developed for phasing large-volume cultures of Escherichia coli K-12 with regard to cell division. The method consists of permitting the bacteria to exhaust a growth-limiting supply of a required amino acid, starving the culture, resupplementing with an excess of the amino acid, and following the ensuing growth by usual counting procedures.
摘要
马特尼，托马斯·S.（得克萨斯大学MD安德森医院和肿瘤研究所，得克萨斯州休斯顿），以及琼·C.休伊特。同步分裂的细菌培养物。一、大肠杆菌中必需氨基酸耗尽和重新补充后的同步化。《细菌学杂志》92:960 - 966。1966年。——开发了一种使大肠杆菌K - 12大量培养物在细胞分裂方面同步化的方法。该方法包括让细菌耗尽所需氨基酸的生长限制供应，使培养物饥饿，用过量的氨基酸重新补充，然后通过常规计数程序跟踪随后的生长情况。
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