双嘧达莫对体外刺激淋巴细胞的胸苷掺入及表面活化抗原表达的差异作用。

Differential effect of dipyridamole upon thymidine incorporation and expression of surface activation antigens by in vitro stimulated lymphocytes.

作者信息

Higuet N, Brohée D

机构信息

Laboratoire de Médecine Expérimentale (Université Libre de Bruxelles), C.H.U. André Vésale, Montigny-le-Tilleul, Belgium.

出版信息

Int J Immunopharmacol. 1992 Aug;14(6):1107-11. doi: 10.1016/0192-0561(92)90156-f.

DOI:10.1016/0192-0561(92)90156-f

PMID:1428366

Abstract

Dipyridamole inhibits, by 50% at 10(-6) M and 100% at 10(-5) M, the incorporation of tritiated thymidine by lymphocytes stimulated with 1 microgram/ml phytohaemagglutinin A for 72 h. At the latter concentration, a clinically relevant one, dipyridamole fails to inhibit lymphocyte proliferation and expression of the cell surface stimulation markers, CD25 and HLA-DR, or differential regulation of the CD45 -RA and -RO isoforms, as determined by single laser two-colour flow-cytometry. No significant immunodepressant effect of dipyridamole can be demonstrated in vitro.

摘要

双嘧达莫在浓度为10⁻⁶M时可抑制50%，在浓度为10⁻⁵M时可完全抑制经1微克/毫升植物血凝素A刺激72小时的淋巴细胞对氚标记胸腺嘧啶核苷的掺入。在后者这个临床相关浓度下，通过单激光双色流式细胞术测定，双嘧达莫未能抑制淋巴细胞增殖、细胞表面刺激标志物CD25和HLA-DR的表达，也未能抑制CD45-RA和-RO同种型的差异调节。在体外未显示双嘧达莫有显著的免疫抑制作用。