Norazmi M N, Arifin H, Jamaruddin M A
Department of Immunology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan.
Immunol Cell Biol. 1995 Jun;73(3):245-8. doi: 10.1038/icb.1995.40.
The lymphocyte subset expressing the gamma delta T cell receptor is increased in several infectious diseases including HIV infection. In this study the expression on gamma delta lymphocytes of the T cell activation markers CD25, HLA-DR and CD38, as well as the two isoforms of CD45, namely CD45RA and CD45RO, was determined in the peripheral blood of 56 HIV-infected intravenous drug users and 34 HIV-seronegative blood donors by two-colour flow cytometry. The percentage of gamma delta lymphocytes expressing HLA-DR and CD38 was higher than those in HIV-seronegative controls (P < 0.001 and P < 0.0001, respectively). Furthermore the HLA-DR+gamma delta+ lymphocytes correlated inversely with CD4+ T lymphocyte absolute count (P < 0.02 for both). The levels of gamma delta lymphocytes expressing CD25, CD45RA and CD45RO were similar to those in HIV-seronegative controls. Activated gamma delta lymphocytes may play a role in the HIV disease process and could provide a useful marker for disease progression.
表达γδT细胞受体的淋巴细胞亚群在包括HIV感染在内的多种传染病中会增加。在本研究中，通过双色流式细胞术测定了56名感染HIV的静脉吸毒者和34名HIV血清阴性献血者外周血中γδ淋巴细胞上T细胞活化标志物CD25、HLA-DR和CD38的表达，以及CD45的两种同种型，即CD45RA和CD45RO的表达。表达HLA-DR和CD38的γδ淋巴细胞百分比高于HIV血清阴性对照组（分别为P < 0.001和P < 0.0001）。此外，HLA-DR+γδ+淋巴细胞与CD4+T淋巴细胞绝对计数呈负相关（两者均为P < 0.02）。表达CD25、CD45RA和CD45RO的γδ淋巴细胞水平与HIV血清阴性对照组相似。活化的γδ淋巴细胞可能在HIV疾病进程中起作用，并可能为疾病进展提供有用的标志物。
