REIMANN B E, LEWIN J C, VOLCANI B E
J Cell Biol. 1965 Jan;24(1):39-55. doi: 10.1083/jcb.24.1.39.
An electron microscope study on the cell wall of the diatom Cylindrotheca fusiformis was carried out using stereoscopic and sectioning techniques. Material prepared by an enzyme treatment or by a mechanical method showed that the wall consists of two major components: a silica shell and organic material. Vapor of hydrofluoric acid was employed to remove the silica and thereby reveal the arrangement of the organic material. An attempt was made to increase the contrast of the organic component by "staining." Uranylacetate not only increased the electron opacity of the organic material but also apparently decreased the electron opacity of the silica shell. In ultrathin sections of complete cells, the structure as revealed by stereoscopy could be confirmed and extended. Every part of the silica shell is tightly enclosed by organic material. In the valve region the silica enclosed in this way is located between other layers of organic material. The whole cell wall is surrounded by a mucilaginous substance which stains with ruthenium red.
利用立体显微镜和切片技术对梭形筒柱藻的细胞壁进行了电子显微镜研究。通过酶处理或机械方法制备的材料表明，细胞壁由两个主要成分组成：硅质壳和有机物质。使用氢氟酸蒸汽去除硅质，从而揭示有机物质的排列。试图通过“染色”来增加有机成分的对比度。醋酸铀不仅增加了有机物质的电子不透明度，而且明显降低了硅质壳的电子不透明度。在完整细胞的超薄切片中，可以证实并扩展立体显微镜所揭示的结构。硅质壳的每个部分都被有机物质紧密包围。在瓣膜区域，以这种方式封闭的硅质位于其他有机物质层之间。整个细胞壁被一种用钌红染色的粘液物质所包围。
