Clarke R, Naughten E, Cahalane S, Sullivan K O, Mathias P, McCall T, Graham I
Department of Cardiology, Adelaide Hospital, Dublin.
Ir J Med Sci. 1992 Sep;161(9):561-4. doi: 10.1007/BF02940559.
Hyperhomocysteinemia has been suggested as a potent new risk factor for premature cardiovascular disease. Homocsyteine can induce endothelial cell injury but the mechanism is not understood. The purpose of this study was to evaluate the role of free radicals as potential causes of endothelial cell injury in a case-control study of obligate heterozygotes for cystathionine beta-synthase deficiency. Firstly, free radical production as measured by neutrophil chemiluminescence in obligate heterozygotes for cystathionine beta-synthase deficiency was compared with age- and sex-matched normal subjects. Secondly, the response of the cellular antioxidant system was examined by measuring the enzymes superoxide dismutase and glutathione peroxidase, their cofactors (selenium, copper), vitamin E and vitamin A in heterozygotes and normal subjects. Analyses of neutrophil chemiluminescence, vitamin A and E, glutathione peroxidase, selenium and copper showed no difference between heterozygotes and controls. While superoxide dismutase activity was higher in heterozygotes than normal subjects, the difference did not reach statistical significance and the hypothesis of excess free radical production as a mechanism of injury was not confirmed. However, further examination of superoxide dismutase activity in a larger number of subjects would be of interest.
高同型半胱氨酸血症已被认为是早发性心血管疾病的一种新的重要危险因素。同型半胱氨酸可诱导内皮细胞损伤，但其机制尚不清楚。本研究的目的是在一项关于胱硫醚β-合酶缺乏症的 obligate 杂合子的病例对照研究中，评估自由基作为内皮细胞损伤潜在原因的作用。首先，将胱硫醚β-合酶缺乏症的 obligate 杂合子中通过中性粒细胞化学发光测定的自由基产生与年龄和性别匹配的正常受试者进行比较。其次，通过测量杂合子和正常受试者中的超氧化物歧化酶和谷胱甘肽过氧化物酶、它们的辅助因子（硒、铜）、维生素E和维生素A，来检查细胞抗氧化系统的反应。中性粒细胞化学发光、维生素A和E、谷胱甘肽过氧化物酶、硒和铜的分析显示杂合子与对照组之间没有差异。虽然杂合子中的超氧化物歧化酶活性高于正常受试者，但差异未达到统计学意义，作为损伤机制的自由基产生过多的假设未得到证实。然而，在更多受试者中进一步检查超氧化物歧化酶活性将是有意义的。
