Dhoot G K
Department of Basic Sciences, Royal Veterinary College, London, UK.
Histochemistry. 1992 Jul;97(6):479-86. doi: 10.1007/BF00316067.
Three monoclonal antibodies (LM5, F2 and F39) to the fast class of myosin heavy chain (MHC) were used to study the effect of denervation on the differentiation of muscle cell types in some rat skeletal muscles. Antibody LM5 in immunocytochemical investigations did not stain any myotubes during early fetal development but presumptive fast muscle cells started to stain during later fetal development. Unlike antibody LM5, antibodies F2 and F39 stained all myotubes during fetal development. The suppression of fast myosin heavy chains recognised in presumptive slow muscle cells was observed within 1-2 days after birth with antibody F39 but not until 10-14 days after birth with antibody F2. The emergence of subsets of fast muscle fibre types in rat extensor digitorum longus (EDL) and tibialis anteri (TA) detectable by F39 and F2 antibodies was not observed until 2-3 weeks after birth. Denervation of developing muscles led to marked changes in the expression of myosins identified by these antibodies.
三种针对肌球蛋白重链（MHC）快速类型的单克隆抗体（LM5、F2和F39）被用于研究去神经支配对部分大鼠骨骼肌中肌肉细胞类型分化的影响。在免疫细胞化学研究中，抗体LM5在胎儿发育早期未对任何肌管进行染色，但在胎儿发育后期，推测的快肌细胞开始被染色。与抗体LM5不同，抗体F2和F39在胎儿发育期间对所有肌管进行了染色。出生后1 - 2天，用抗体F39可观察到推测的慢肌细胞中快肌球蛋白重链受到抑制，但用抗体F2则直到出生后10 - 14天才观察到。直到出生后2 - 3周，用F39和F2抗体才可检测到大鼠趾长伸肌（EDL）和胫骨前肌（TA）中快肌纤维类型亚群的出现。发育中的肌肉去神经支配导致这些抗体所识别的肌球蛋白表达发生显著变化。
